异体神经干细胞脑移植后免疫排斥反应的研究

Immunological rejection in brain of cloning goats received neural stem cell transplantation

目的 探讨中枢神经系统异体神经干细胞移植后免疫排斥反应的问题. 方法 选取同一批次的成年克隆山羊8只,采用随机数字表法分成2组：神经干细胞移植组和对照组.采用开颅手术的方式分别将异体神经干细胞及相同剂量的生理盐水移植到山羊脑皮层下,抽血测定移植后2组动物不同时间点（移植前1周、移植当时、移植后1周、3周及3个月）血中IL-2及IL-10的水平,以了解移植后动物全身免疫排斥反应的情况;并通过免疫组化的方法 检测移植局部病理切片CD3＋细胞浸润的情况,了解移植局部免疫排斥反应的情况. 结果神经干细胞移植组在移植后1周、3周及3个月时血中IL-2水平较对照组明显升高,IL-10水平较对照组明显下降,差异均有统计学意义（P＜0.05）.移植局部在急性期（细胞移植后1周）和慢性期（细胞移植后3个月）两个时间点均有大量CD3＋细胞浸润,与对照组相比差异均有统计学意义（P＜0.05）. 结论 中枢神经系统异体神经干细胞移植后可发生急、慢性全身和局部免疫排斥反应.

Objective To investigate the immunological rejection in the brain of cloning goats received neural stem cell transplantation. Methods Eight cloning goats of CL series were chosen at random and divided into 2 groups. Neural stem cells and saline at the same dosages were transplanted into the fixed site by surgical intervention in the brain cortex of each group, respectively. The levels of IL-2 and IL-10 in the blood of each group were detected at different times （1 w before, and 0, 1 and 3 w,and 3 months after the cell transplantation） to reflect the systemic immune rejection of the goats after the transplatation. The CD3＋ cells in the cell transplantation areas in each group were also detected by the method of immunohistochemistry to reflect the local immune rejection after the transplatation. Results The level of IL-2 was obviously higher and the level of IL-10 was obviously lower in the neural stem cell transplantation group than those in the control group 1 and 3 w, and 3 months after the cell transplantation （P＜0.05）. The quantity of CD3＋ cells in the neural stem cell transplantation group was much larger than that of control groups at the acute period （1 w after cell transplantation） and chronic period （3 months alter cell transplantation, P＜0.05）. Conclusion Systemic and local immunological rejections at acute or chronic periods will appear at the brain of cloning goats with neural stem cells transplantation.