摘要
体细胞重编程与microRNAs(miRNAs)均为近年来研究的热点问题。到目前为止,能成功诱导体细胞形成多能性干细胞的体细胞重编程方法有核移植(nuclear transfer,NT)和外源因子诱导形成多能干细胞(induced pluripotent stem cells,iPSc)两种,这两种方法让人们看到了体细胞重编程在细胞治疗方面具有诱人的应用前景。miRNAs是真核生物中存在的一类长度为22nt左右起调控作用的内源性非编码RNA,它在转录后水平调节靶基因的表达,是细胞内基因表达的基本调控机制之一。近年的研究结果表明,miRNAs在干细胞干性维持和分化过程中具有重要的调节作用,从miRNAs角度研究体细胞重编程机理将对体细胞重编程的应用具有重要意义。
The research about somatic reprogramming and microRNAs (miRNAs) has received widespread attention in clinical and basic research. Adult cells can be successfully reprogrammed into pluripotent stem cells by nuclear transfer (NT) and induced pluripotent stem (iPS) cells. NT and iPS techniques offer tremendous promises for cell therapies. MiRNAs are small (~22 nucleotides) non-coding RNAs that play important role in posttranscriptional gene regulation. MiRNAs involved in maintaining self-renewal and differentiation of stem cells. Understanding of the mechanism of miRNAs in somatic reprogramming will provide theoretical basis on its clinical application.
出处
《生命科学》
CSCD
北大核心
2010年第7期674-681,共8页
Chinese Bulletin of Life Sciences
基金
中科院知识创新工程重要方向性项目(KSCX2-YW-R-110)(KSCX2-YW-R-229)