酶联免疫分析法测定水产品中氯霉素残留量

Determination of chloramphenicol residues in aquatic products by enzyme immnunoassay assay

目的:建立测定水产品中氯霉素残留量的ELISA分析法。方法:对不同样品采用不同的前处理方法,用ELISA方法进行检测。结果:方法检测限为0.01μg/kg;在0.5μg/kg和2.0μg/kg两个添加水平,样品加标平均回收率分别为72%～116%和86%～108%;重复性实验:RSD分别为1.8%～6.2%和6.5%～7.8%;重现性实验RSD分别为3.8%和5.9%。结论:本方法灵敏度、准确度和精密度均符合残留分析质量控制的要求,适用于水产品中氯霉素残留量的快速检测。

Objective：To develop an enzyme immunoassay method for the detection of chloramphenicol（CAP） residues in aquatic products.Methods：CAP residues in different samples were extracted with different methods and detected an enzyme immunoassay kit detected.Results：The limit of detection（LOD） was 0.01 μg/kg.On the spiked levels of 0.5 μg/kg and 2.0 μg/kg,the mean recovery of ths method were 72%～116% and 86%～108%,respectively,with the RSD foe the repeatability of 1.8%～6.2% and 6.5%～7.8%,and the RSD for the reproducibility of 3.8% and 5.9%.Conclusion：The sensitivity,accuracy and precision of the method are consistent with the guide for quality control of residues analysis.It is suitable for the rapid detection of CAP residues in aquatic products.