芴降解优势菌的筛选鉴定及降解特性研究

Screening,identification and degradation characteristics of a dominant fluorene-degrading strain

从某焦化厂活性污泥中分离筛选出一株能以芴为碳源和能源生长的细菌(命名为W-2),在形态学观察和生理生化试验基础上,利用16S rDNA序列分析及系统发育学分析的方法,鉴定菌株W-2为微嗜酸寡养单胞菌(Stenotrophomonas acidaminiphila).考察了菌株W-2在液体培养基体系内对芴的降解效果,结果表明,该菌株对芴具有良好的降解特性,在初始芴浓度为40mg/L,接种量10%(V/V),pH7.0,温度30℃条件下,接种该菌11d后,芴的降解效率达到86.0%,说明该菌在芴污染控制方面具有良好的应用前景.

A dominant strain（named W-2） which can utilize fluorene as carbon and energy source for growth was isolated and screened from activated sludge of a coking plant.On the basis of morphological observation and physio-biochemical test,the strain W-2 was identified as Stenotrophomonas acidaminiphila by 16S rDNA gene sequence and phylogeny analysis.The biodegradation efficiency of fluorene by strain W-2（S.acidaminiphila） in solution culture was further investigated.Results indicated that the strain could effectively degrade fluorene and the biodegradation efficiency reached 86.0%after 11 days under the conditions of initial fluorene concentration 40mg/L,inoculation amount 10%（V/V）,pH 7.0 and temperature 30℃.The results showed that strain W-2 had a good application prospect in control of fluorene pollution.