微图案化钙磷盐膜层的电化学构筑及其生物性能

Electrochemical Construction and Biological Performance of Micropatterned CaP Films

基于表面分子自组装和光催化转印技术,在TiO2膜层表面获得超亲/超疏水阵列微图案模板,结合电化学沉积技术,成功制备了微图案化钙磷盐膜(CaP)层.扫描电子显微镜(SEM)和电子探针分析(EPMA)结果表明,通过超亲/超疏水阵列微图案模板可构筑高空间分辨的微图案化钙磷盐膜层.微图案化钙磷盐膜层的体外MG-63细胞培养证实,细胞对钙磷盐膜层微单元有强烈的选择性粘附作用,从而可望控制细胞在微单元中的贴壁生长,实现高通量评价细胞行为.

Based on surface molecule self-assembly and photocatalytic lithography techniques, superhydrophilic/ superhydrophobic micropatterns were fabricated on TiO2 films. Micropatterned calcium phosphate （CaP） films were successfully fabricated by the as-prepared superhydrophilic/superhydrophobic template combined with the electrochemical deposition method. Scanning electron microscopy （SEM） and electron probe microanalysis （EPMA） indicated that micropatterned CaP films with a high spatial resolution could be constructed using the superhydrophilic/ superhydrophobic micropatterns as templates. In vitro MG-63 cell tests of the micropatterned CaP films showed that the cells selectively adhered to the tiny CaP film units, which is promising for the control of the adherent growth of the cells on the tiny units and to achieve a high throughput evaluation of the cell behavior.