吗啡对电刺激隐神经诱发大鼠扣带回前部神经元c-fos基因表达的影响

Effect of Morphine on c-fos Gene Expression Induced by Electrical Stimulation of Saphenous Nerve in the Anterior Cingulate Gyrus Neurons of Rats

目的观察躯体伤害性刺激能否引起大鼠扣带回前部(ACG)神经元c-fos基因表达的改变,探讨吗啡对该变化的影响。方法用免疫组化方法研究伤害性电刺激隐神经(SN)后不同时间ACG神经元c-fos基因表达的变化以及皮下注射吗啡对该变化的影响。结果伤害性电刺激SN后30minACG神经元Fos蛋白表达明显增加,60min增加最明显,120min后开始消退;皮下注射吗啡抑制了伤害性电刺激SN引起的ACG神经元Fos蛋白表达的显著增加。结论伤害性电刺激SN能够引起ACG神经元Fos蛋白表达的显著增加,这种表达呈时间依赖性;吗啡抑制了伤害性电刺激SN引起的ACG神经元Fos蛋白表达的显著增加。提示ACG存有SN代表区,能够感受SN传入的伤害性信息。

Objective To investigate whether noxious somatic stimulation could induce changes in c-fos gene expression in the anterior cingulate gyrus（ACG）neurons and the effect of morphine on the changes.Methods The expression of Fos protein in ACG neurons was detected by immunohistochemistry at different times after electrical stimulation of saphenous nerve（SN）,and the effect of subcutaneously injected morphine on the expression of Fos protein was determined.Results The expression Fos protein in ACG neurons significantly increased 30 minutes after the electrical stimulation of SN,reached the peak after 60 minutes,and began to decrease after 120 minutes.After subcutaneous injection of morphine,the increased expression of Fos protein induced by noxious electrical stimulation in ACG neurons was inhibited.Conclusion Noxious electrical stimulation of SN could increase the expression of Fos protein in ACG neurons in a time-dependent manner,and the increased expression of Fos protein is inhibited by morphine,suggesting that ACG is one of the representative areas of SN and can receive the nociceptive afferent information from SN.