摘要
目的观察躯体伤害性刺激能否引起大鼠扣带回前部(ACG)神经元c-fos基因表达的改变,探讨吗啡对该变化的影响。方法用免疫组化方法研究伤害性电刺激隐神经(SN)后不同时间ACG神经元c-fos基因表达的变化以及皮下注射吗啡对该变化的影响。结果伤害性电刺激SN后30minACG神经元Fos蛋白表达明显增加,60min增加最明显,120min后开始消退;皮下注射吗啡抑制了伤害性电刺激SN引起的ACG神经元Fos蛋白表达的显著增加。结论伤害性电刺激SN能够引起ACG神经元Fos蛋白表达的显著增加,这种表达呈时间依赖性;吗啡抑制了伤害性电刺激SN引起的ACG神经元Fos蛋白表达的显著增加。提示ACG存有SN代表区,能够感受SN传入的伤害性信息。
Objective To investigate whether noxious somatic stimulation could induce changes in c-fos gene expression in the anterior cingulate gyrus(ACG)neurons and the effect of morphine on the changes.Methods The expression of Fos protein in ACG neurons was detected by immunohistochemistry at different times after electrical stimulation of saphenous nerve(SN),and the effect of subcutaneously injected morphine on the expression of Fos protein was determined.Results The expression Fos protein in ACG neurons significantly increased 30 minutes after the electrical stimulation of SN,reached the peak after 60 minutes,and began to decrease after 120 minutes.After subcutaneous injection of morphine,the increased expression of Fos protein induced by noxious electrical stimulation in ACG neurons was inhibited.Conclusion Noxious electrical stimulation of SN could increase the expression of Fos protein in ACG neurons in a time-dependent manner,and the increased expression of Fos protein is inhibited by morphine,suggesting that ACG is one of the representative areas of SN and can receive the nociceptive afferent information from SN.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第7期532-534,共3页
Journal of China Medical University
基金
辽宁省科学技术厅基金资助项目(20072064)
教育部留学回国人员科研启动基金(教外司留[2008]101号)
辽宁省教育厅高校科研基金资助项目(2009A698)
沈阳市科学技术局计划项目(1063317-1-00)
关键词
扣带回前部
隐神经
躯体痛
C-FOS基因
免疫组化
anterior cingulate gyrus
saphenous nerve
somatic pain
c-fos gene
immunohistochemistry