不同方法培养小球藻的试验

EXPERIMENT ON DIFFERENT CULTURE METHODS OF CHLORELLA SPP

试验系采用不同方法培养小球藻观察其生长情况。小球藻在水泥地中培养，最高密度是40．0cm组的2880．0×104cells／mL，但培养液深度在20．0～60．0cm范围内生长无显著差异；在三角烧瓶中培养指数生长末期的最高密度达8000．0×104cells／mL；在生物反应器中培养，一次性培养指数生长期末期的最高密度达13000．0×104cells／mL，半连续培养时适宜的起始密度为4500．0×104cells／mL，每天可以采收1／3藻液，密度约为6800．0×104cells／mL，这样每天就有较高的、稳定的收获量。结果表明小球藻在生物反应器中培养其生长最好。

Growth of chlorella spp. cultured by different methods was investigated. The maximum cell density in concrete ponds was 2880.0×104 cells/mL in 40.0cm group of alga culture medium. But there was no significant difference among the growth in the ponds with depth of alga culture media from 20.0 cm to 60.0cm. The growth cultured in conical flasks reached 8000.0×104 cells/mL at the end of exponential phase. The cell density in bio reactor was 13000. 0 × 104 cells/mL. If original cell density was 4500.0×104 cells/mL and 1/3 alga was daily harvested, the growth in bio-reactor might be maintained at 6800.0×104 cells/mL. Therefore the culture of chlorela spp. in bio-reactor was the best method.