舟山、杭州两地淡色库蚊对杀虫剂抗性特征分析

Insecticide resistance of Culex pipiens pallens in Zhoushan and Hangzhou

目的了解浙江省不同地理环境下淡色库蚊对杀虫剂的抗性特征,为蚊虫抗性产生机制以及防制措施的制定提供科学依据。方法采用浸渍法检测淡色库蚊敏感品系、舟山现场种群、杭州现场种群对敌敌畏、溴氰菊酯、三氯杀虫酯、残杀威、丙烯菊酯、高效氯氰菊酯4类6种常用杀虫剂的抗性特征。结果杭州现场种群抗性水平普遍较低,抗性系数在0.78～7.58倍之间,其由高到低依次为敌敌畏(7.58倍)>溴氰菊酯(4.00倍)>三氯杀虫酯(2.88倍)>残杀威(2.11倍)>丙烯菊酯(1.11倍)>高效氯氰菊酯(0.78倍)。舟山现场种群抗性系数在2.25～15.00倍之间,其由高到低依次为溴氰菊酯(15.00倍)>三氯杀虫酯(7.54倍)>敌敌畏(5.99倍)>高效氯氰菊酯(5.57倍)>丙烯菊酯(4.55倍)>残杀威(2.25倍)。淡色库蚊的抗性系数舟山现场种群除敌敌畏外,其余均高于杭州现场种群,其中对高效氯氰菊酯、丙烯菊酯和溴氰菊酯的抗性系数比杭州现场种群高6.14、3.10和2.75倍。结论舟山、杭州现场种群抗性水平普遍较低,舟山现场种群除溴氰菊酯外,两地现场种群对其他药物的抗性系数均<10。两市比较,舟山现场种群除敌敌畏外,其他抗性均略高于杭州现场种群。

Objective To identify the insecticide resistance of Culex pipiens pallens in different habitats of Zhejiang province,providing the basis for study of insecticide resistance mechanisms in mosquitoes and for development of specific prevention and control measures.Methods The impregnation method was employed to measure the resistance of susceptible strains and field strains of Cx.pipiens pallens from Zhoushan and Hangzhou to 6 insecticides of 4 kinds,including dichlorvos,deltamethrin,acetophenate,propoxur,allethrin and beta-cypermethrin.Results The resistance of Hangzhou strains was generally low,with the resistance coefficients ranging from 0.78 to 7.58 times in descending order as follows：dichlorvos（7.58 times） deltamethrin（4.00 times） acetophenate（2.88 times） propoxur（2.11 times） allethrin（1.11 times） beta-cypermethrin（0.78 times）.The resistance coefficients of Zhoushan strains were between 2.25 and 15.00 times in descending order as follows：deltamethrin（15.00 times） acetophenate（7.54 times） dichlorvos（5.99 times） beta-cypermethrin（5.57 times） allethrin（4.55 times） propoxur（2.25 times）.Each of the resistance coefficients of Zhoushan strains of Cx.pipiens pallens was higher than that of Hangzhou strains except for dichlorvos;the resistance coefficients of the former to beta-cypermethrin,allethrin and deltamethrin were 6.14,3.10 and 2.75 times higher than those of the latter.Conclusion The insecticide resistance of Zhoushan and Hangzhou strains was generally low.None of the resistance coefficients of the two strains was higher than 10 to any of the insecticides except deltamethrin.Each of the resistance coefficients of Zhoushan strains was higher than the counterpart of Hangzhou strains except for dichlorvos