摘要
目的:建立测定人血浆中亮菌甲素琥珀酸单酯的高效液相色谱法。方法:色谱柱为DL-C_(18)(150 mm×4.6 mm,5μm),流动相:甲醇-10 mmol乙酸铵水溶液-乙酸(45:55:0.5);柱温25℃,流速1.0ml·min^(-1),检测波长220 nm。结果:亮菌甲素琥珀酸单酯方法最低检测浓度为5 ng·ml^(-1),线性范围0.05～10.00μg·ml^(-1)(r=0.999 6)线性关系良好。亮菌甲素琥珀酸单酯日内RSD分别为2.09%～4.22%,日间RSD分别为3.72%～5.04%。方法回收率分别为94.38%～97.20%。结论:本试验建立的方法简便、快速、准确,适用于亮菌甲素琥珀酸单酯体内血药浓度和生物等效性的测定。
Objective： To establish a method for determing the armillarisin amber acid ester by HPLC. Method： The DL- C18 （ 150 mm ×4. 6 mm,5 μm） column was used with methanol-10 mmol ammonium acetate aqueous-acetic acid （45：55：0. 05） as mobile phase, flow rate was 1.0 ml·min^-1, the column tempreture was 25 ℃ and the wavelength was at 220 ran. Result： The limits of detection were 5 ng·ml^-1. The concentration of armillarisin amber acid ester showed a linear relationship within the range of 0. 05-10. 00 μg · ml^ - 1 （ r = 0. 999 6）. Within-day and between-day RSD for the method were 2.09% -4. 22% and 3.72% -5.04% respectively. The extraction recovery rates were 94.38%-97.20%. Conclusion： The method is proved to be accurate, sensitive and reliable. It is suitable for the determination of armillarisin amber acid ester in human plasma.
出处
《中国药师》
CAS
2010年第8期1092-1094,共3页
China Pharmacist
基金
湖北省教育厅项目(编号:B20092410)
