环境因子对牙鲆精子运动能力的影响

EFFECTS OF ENVIRONMENTAL FACTORS ON SPERM MOTILITY IN FLOUNDER, PARALICHTHYS OLIVACEUS (T. ET S.)

于1997年5-6月,在山东荣成市石岛养殖场采用挤压法收集牙鲆亲鱼的精子,利用显微镜观察精子的活力,研究外界环境因子的变化对牙鲆精子运动能力的影响。结果表明,将蔗糖、NaCl、KCl、MgCl2、CaCl2等溶解于去离子水中,制成不同浓度即具有不同渗透压的溶液,不同渗透压的溶液对精子运动的诱导结果不同。EDTANa2溶液不能诱导精子运动。在人工海水中加入一定量的EDTANa2后,可使原先运动的精子静止;在该溶液中加入新的Ca2+后,将不再诱导原来受到EDTANa2抑制的精子。如用MgSO4代替溶液中的CaCl2,可诱导精子运动;而当用MgCl2代替溶液中的CaCl2,以及用CaCl2或NaCl代替MgSO4时,溶液即失去诱导精子运动的能力。在pH＝4.0-9、5的海水中,牙解精子均可正常运动。

Between May and June in 1997, experiments were conducted to determine the effects of external factors on the sperm motility in flounder, Paralichthys olivaceus (T. et S.). The different solutes, which included sugar, NaCl, KCl, CaCl2, MgCl2, EDTANa2, were dissolved in double distilled water to produce solutions with different concentrations. The sperm could be initiated by increasing the external osmolity. With different solutes, the osmolity levels that could initiate or terminate the sperm motility were different. EDTANa2 had no induction effect. When EUTANa2was added to the artificial seawater, the sperm could not be initiated and the original active sperm was inhibited. When Ca2+ was dissolved in such solution, the sperms tha were inhibited could not motile again. When MgSO4 was dissolved in the solution to replace CaCl2, the sperm could be initiated. But when CaCl2 was replaced by MgCl2, or MgSO4 was replaced by CaCl2 or NaCl, the sperm kept inactive. The sperm could be induced by the seawater with different pH-value ranging between 4.0 and 9.5.