对硝基苯基-β-D-葡萄糖醛酸苷钠盐的合成

Synthesis of sodium p nitrophenyl β D glucuronate

以葡萄糖醛酸内酯和对硝基酚为原料，经过五步反应合成了对硝基苯基－β－Ｄ－葡萄糖醛酸苷钠盐（ＰＮＰ－ＧＵＡ），熔点２２０℃，并用红外和核磁共振予以确认。以ＰＮＰ－ＧＵＡ为底物，在检测环境样品的大肠杆菌中，得到满意的结果。

Sodium p nitrophenyl β D glucuronate(PNP GUA) was synthesized in five steps from D glucuronolactone and p nitrophenol,mp220℃.As a substrate it was successfully applied to the detection and enumeration of Escherichia coli in environmental samples.