乙型肝炎病毒大蛋白的临床应用分析研究

Large Serum Protein of Hepatitis B Virus and Its Clinical Application Analysis

目的通过检测血清样本中HBVDNA、乙型肝炎病毒大蛋白(LHBs)、乙型肝炎五项指标以及乙型肝炎前S1(PreS1Ag),探讨LHBs用于乙型肝炎临床诊断的价值及与其他乙型肝炎病毒标志的相关性。方法收集623份乙型肝炎患者血清标本,LHBs、乙型肝炎五项指标以及PreS1Ag,采用ELISA技术检测了HBsAg阳性血清标本中的LHBs、HbeAg、PreS1Ag抗原,采用荧光定量PCR技术检测HBVDNA。结果 515份HBVDNA阳性血清标本中,LHBs检测阳性485份(94.17%);PreS1Ag检测阳性260份(50.49%);LHBs阳性率明显高于PreS1Ag阳性率,两者差异有统计学意义(P<0.01);在检测270份HBeAg阴性血清标本中,HBVDNA检出阳性133份(49.26%),LHBs检出阳性119份(44.07%),两者差异无统计学意义;LHBs吸光度(OD值)与HBVDNA呈正相关关系(r=0.980)。结论 LHBs可用于反映乙型肝炎病毒复制水平的敏感新指标,操作方法便捷适合临床应用。

OBJECTIVE To explore the value of large serum protein of hepatitis B virus （LHBs） in diagnosing type of hepatitis B and the pertinency with other markers of HBV.METHODS The enzyme linked immunosorbent assay （ELISA ） methods were used to examine LHBs,while Heat,Pres1Ag,and fluorescent quantitative PCR were used to detect the HBV DNA of 623 HBsAg positive serum samples.RESULTS The positive rates of LHBs and PreS1Ag were respectively 94.17% and 50.49%,the difference was statistically significant.Of the 270 HBeAg negative samples,the positive rates of HBV DNA and LHBs were respectively 49.26% and 44.07%,there was without statistical significance.LHBs optical density was positively correlated with HBV DNA copy level.CONCLUSION LHBs can be used as a new sensitivity indicator for reflecting the replication of HBV,its convenient operation is suitable for clinical application.