小鼠放射状胶质细胞和小脑皮质的发育

Radial glial cell and development of cerebellar cortex in mouse

目的探讨小鼠小脑皮质发育过程中放射状胶质细胞的分化。方法应用免疫荧光及5-溴脱氧尿嘧啶核苷(BrdU)检测技术,标记小鼠胚胎8d至生后180d小脑(57例,分为19组,每组3只)的神经干细胞、放射状胶质细胞、普肯耶细胞及颗粒细胞。结果放射状胶质细胞于胚胎13d的神经上皮出现,尔后该细胞分化为各种神经元和贝格曼胶质细胞,并在小脑皮质层状结构的形成中起着重要作用。结论放射状胶质细胞来源于神经上皮细胞,是神经细胞和神经胶质细胞的前体细胞。在小脑皮质的发育过程中,放射状胶质细胞能分化为普肯耶细胞和颗粒细胞,并为神经细胞的迁移提供路径和支架。

Objective To investigate the differentiation of radial glial cells during the development of mouse cerebellar cortex. Methods The immunofluoreseent stainings and 5-bromodeoxyuridine（BrdU） assay were used to label neural stem cells, radial glial cells, Purkinje cells and granule cells in cerebellum （57 cases, which were divided into 19 groups,and each had 3 mice） from embryonic day 8 （E8） to postnatal day 180 （P180）. Results The radial glial cells could be found in neuroepithelium at E13. Then they differentiated various neurons and Bergmann cells. The radial glia cells played very important role in the cerebellar lamination. Conclusion Radial glial cells are generated from the neuroepithelial cells, and they act as the neural progenitors for neurons and neuroglia. During the development of cerebellar cortex, radial glial cells can differentiate into Purkinje cells and granule cells and provide the pathways and scaffolds for the neuron migration.