膜联蛋白A7低表达对人宫颈癌HeLa细胞系增殖与凋亡的影响

Influence of annexin A7 low expression on proliferation and apoptosis of HeLa cells

目的探讨膜联蛋白A7表达减少是否对人宫颈癌HeLa细胞系的增殖和凋亡产生影响。方法采用Western blotting鉴定siRNA可否有效抑制膜联蛋白A7表达,然后用脂质体转染法将siRNA转染入HeLa细胞,将细胞分为siRNA干扰组、阴性对照组和空白对照组,分别在转染后48h进行刃天青实验以观察细胞增殖状况的改变;流式细胞术检测3组细胞凋亡情况。结果在转染膜联蛋白A7的siRNA后48h的HeLa细胞,刃天青实验可见siRNA干扰组细胞增殖较阴性对照组和空白对照组显著降低(P<0.05);流式细胞术实验可见siRNA干扰组细胞凋亡显著增多(P<0.05)。结论膜联蛋白A7的低表达可能影响HeLa细胞的增殖和凋亡。

Objective To study whether annexin A7 （ANXA7） low expression can affect apoptosis and proliferation of HeLa cells. Methods Western blotting was used to identify whether siRNA can highly surpress annexin A7 expressin. When the effect of the siRNA was assured, the siRNA was transfected into HeLa cells by lipofeetamine 2000. At the same time, cells in negative control group were transfected negative siRNA while cells as blank control group did not receive any treatment. After transfection for 48 hours, resazurin assay was conducted and DNA content was analyzed by eytometry. Results Resazurin assay showed that proliferation of the siRNA interference cell group decreased significantly （P 〈 0. 05 ）. Cytometry test showed that apoptosis of the siRNA interference group increased obviously （ P 〈 0.05 ）. Conclusion ANXA7 low expression may affect proliferation and apoptosis of HeLa cells.