摘要
为了采用电穿孔法提高酵母菌细胞的通透性,将通过离心得到的酵母菌制成1×108CFU/mL的菌悬液,采用不同的方波电脉冲条件对其进行处理。结果表明:在0.6mol/L的蔗糖等渗电脉冲介质中,当酵母菌细胞的处理条件为电场强度7kV/cm,脉冲宽度50μs,脉冲数14,间隔时间2s时,细胞的存活率平均为81.16%,电穿孔率平均为65.14%。这表明电穿孔法可以有效增强酵母菌细胞的通透性。
Yeast（Saccharomyces cerevisiae CICC 1012） cells were statically cultured in YEPD medium at 30 ℃ for 24 h and then separated by centrifugation at 3000 r/min after the end of culture and suspended in an electric pulse medium（containing sucrose,Tris-HCl and MgCl2,at pH 7.5） at a cell concentration of 1×108 CFU/mL before square-wave electric pulse treatment under different operating conditions.Electric field strength of 7 kV/cm,pulse duration of 50μs,pulse number of 14,time interval of 2 s,and sucrose concentration of 0.6 mol/L were found optimum.The percentage survival of yeast cells was 81.16% and the percentage of electroporated yeast cells was 65.14% under these optimum conditions.This study suggests that the permeability of yeast cells can be increased by square-wave electroporation.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第15期50-54,共5页
Food Science
基金
黑龙江省"十一五"科技攻关计划项目(GA06B402-86-6)
关键词
方波脉冲
电穿孔法
酵母菌
通透性
square-wave electric pulse
electroporation
yeast
permeability
