羊软骨Ⅱ型胶原蛋白对小鼠佐剂性关节炎抑制作用的研究

Inhibitory Effect of Type II Collagen from Sheep Cartilage on Adjuvant Arthritis in Mice

目的:探讨灌胃Ⅱ型胶原蛋白(CⅡ)对佐剂性关节炎小鼠体质量和足底厚度的影响。方法:通过预实验,筛选出0.2mol/LCⅡ的最佳灌胃剂量为0.6mL/d;然后将小鼠随机分为4组:正常组、预防组、治疗组和模型组进行正式实验;最后测定致炎前后不同时期各组小鼠的体质量和足底厚度。结果:预防组和治疗组小鼠体质量呈先减少后增加趋势,小鼠足底厚度分别从致炎12d和14d后呈明显降低趋势,且前者效果优于后者,模型组小鼠致炎后体质量增长受到抑制且足底厚度明显增加。结论:灌胃羊软骨CⅡ能明显缓解佐剂性关节炎小鼠的炎症反应。

s：Objective：To investigate the effect of intragastric administration of collagen II from sheep cartilage on the body weight and plantar thickness in mice with adjuvant arthritis.Methods：The optimal dosage of collagen II was screened using preliminary experiments.Mice were randomly divided into four groups,designated as control group（A）,prevention group（B）,treatment group（C） and model group（D）.The body weight and plantar thickness in mice were measured at different time points before and after induction of inflammation.Results：The plantar thicknesses of groups B and C both began to markedly decrease from 12 and 14 days after inflammatory induction,respectively;the change trend of body weight initially decreased,followed by an increase.Group B was superior to group C in body weight recovery.However,body weight was inhibited but plantar thickness increased significantly after inflammatory induction in group D.Conclusion：Intragastric administration of collagen II can relieve inflammatory reaction in mice with adjuvant arthritis.