甘油三酯体外抗肿瘤活性(英文)

Inhibitory Effect of Triacylglycerols on Cultured Human Tumor Cells

经非水反相高效液相色谱(NARP-HPLC)纯化,从癞葡萄籽油(MCVO)中分离得到了6个含共轭亚麻酸(CLns)的甘油三酯(TGs)组分。以纯度为96%的α-桐酸(α-ESA)为对照,考察甘油三酯组分对5个人体肿瘤细胞株(ECA-109、SGC-7901、Bel-7402、LS-174-T、KB)和1个人体正常细胞株(L02)的生长抑制效果。结果显示含CLn的TG组分对人体肿瘤细胞的抑制作用远强于α-ESA,而且由3个CLns组成的TG是TG组分中效果最弱的。结果表明:TG的脂肪酸组成和TG的分子结构(可使CLn稳定性提高)对含CLn的TG组分抑制人体肿瘤细胞的生长具有重要影响。

Six fractions of triacylglycerols（TGs） containing conjugated linolenic acids（CLns） were separated from the seed oil of Momordica charantia L.var.abbreviata Ser.（MCVO） and purified by non-aqueous reversed-phase（NARP）-HPLC.The cytotoxic effects of the fractions were further investigated on five human tumor cell lines：ECA-109（esophageal carcinoma）,SGC-7901（carcinoma ventriculi）,Bel-7402（hepatoma）,LS-174-T（colon carcinoma）,KB（mouth epidermal carcinoma）,and a human normal cell line：L02（liver cell）.α-Eleostearic acid（α-ESA） with a purity of 96% was used as a control.The results demonstrated that the inhibitory effects of the TG fractions on human tumor cell lines were more potent than that of α-ESA,and the effect of TG with three CLns was the weakest.These results suggest that the fatty acid compositions and molecular structures of the TG with more intense stability,which contain CLn,might affect the displayed cytotoxicity to human tumor cell lines.