反相离子对高效液相色谱法分离和测定铝、镓和铟

Separation and Determination of Microamounts of Aluminium,Gallium and Indium by High Performance Liquid Chromatography

以芪－４，４′－双（１－偶氮）－３，４－二羟基苯－２，２′－二磺酸铵（芪唑）为柱前衍生试剂，采用反相离子对高效液相色谱法分离和测定铝（Ⅲ）、镓（Ⅲ）和铟（Ⅲ）。在ｓｐｈｅｒｅｘＣ１８柱上，用浓度为１２ｍｍｏｌ／Ｌ的ＳＤＳ，浓度为２０ｍｍｏｌ／ＬｐＨ３．５的ＡＨｃ－ＮａＡｃ的甲醇－水（体积比为６０∶４０）溶液作流动相，检测波长为５０６ｎｍ。同时分离和测定了３种金属络合物。铝（Ⅲ）、镓（Ⅲ）和铟（Ⅲ）离子的检出限分别为１４μｇ／Ｌ、２３μｇ／Ｌ和２８μｇ／Ｌ，方法用于矿石样品分析。

The reversed phase high performance liquid chromatography spectrophotometric determination of aluminium,gallium and indium with diammonium stilbene 4,4′ bis (1 azo) 3,4 dihydroxybenzene 2,2′ disulfate,as precolumn chelate reagent on Spherex C 18 column has been investigated.The mobile phase is methanol water (60∶40, V/V ) containing 20mmol/L pH3.5 acetate buffer and 12mmol/L sodium dodecyl sulfonate (SDS).The 3 complexes can be separated completely within 16min.The detection limits are 14μg/L,23μg/L and 28μg/L for aluminium(Ⅲ),gallium(Ⅲ) and indium(Ⅲ) ,respectively,when the ratio of signal to noise is 3.The method has been applied to the determination of aluminium,gallium and indium in samples.