黄芪、当归对血管再狭窄大鼠超声血流动力学的影响

Effect of Astragalus membranaceus and Angelica sinensis on the hemodynamics of restenosis of blood vessel in rat

目的研究中药当归、黄芪对内皮剥脱后血管再狭窄大鼠病理形态及超声血流动力学变化的影响,探讨黄芪、当归防治血管再狭窄的作用机制。方法健康雄性SD大鼠30只,随机均分为5组(n=6):对照(C)组、模型(M)组、黄芪治疗(H)组、当归治疗(D)组和黄芪当归联合治疗(HD)组。除C组外,其他各组均建立大鼠内皮剥脱后再狭窄动物模型,H、D和HD组分别应用黄芪、当归注射液单独或联合治疗21d,观察大鼠血管病理形态,并采用彩色多普勒超声检查血流动力学指标的变化情况。结果与C组比较,M组大鼠的主动脉壁明显增厚,血流速度明显减低,阻力指数及搏动指数升高;与M组比较,H、D及HD组主动脉壁厚度明显减轻,血流速度加快,阻力指数及搏动指数降低。结论黄芪、当归不仅可抑制内皮损伤所致的内膜增厚,还可通过改善超声血流动力学指标发挥防治血管再狭窄的作用。

Objective To investigate the effects of Astragalus membranaceus and Angelica sinensis on the hemodynamics and pathological features of arterial wall in restenosis of blood vessel in rats in order to find the potential mechanism of the effect of Astragalus membranaceus and Angelica sinensis in preventing restenosis after ablation of intima. Method Thirty SD rats were randomly divided into 5 groups （6 each）：control group （C）,model group （M）,Astragalus membranaceus treatment group （H）,Angelica sinensis treatment group （D）,and combined Astragalus membranaceus with Angelica sinensis treatment group （HD）. The restenosis model was reproduced by denuding rat＇s aorta endothelium with balloon catheter in all the groups except group C. After intramuscular injection of Astragalus membranaceus 0.42ml/（kg·d） in group H,Angelica sinensis 0.21ml/（kg·d） in group D,and Astragalus membranaceus 0.42ml/（kg·d） together with Angelica sinensis 0.21ml/（kg·d） in group HD for 21 days,the changes in hemodynamics and the pathological features of arterial wall were observed in all the groups. Result Compared with rats of group C,in which the intima was smooth and the smooth muscle cells （VSMCs） in media were orderly arranged,the intimal thickness of aorta,pulsatile index （PI） and resistance index （RI） increased,while the diameter of lumen,blood velocity decreased remarkably in group M. Compared with the rats in group M,the extent of hyperplasia and thickness was notably reduced,RI and PI were lower while blood velocity was lowered in group H,D and HD after being treated for 21 days. Conclusion Hyperplasia induced by remaval of endothelial cells may be reduced and blood circulation may be improved to different extents by individually or simultaneously use of Astragalus membranaceus and Angelica sinensis,thus preventing restenosis of the aorta after removal of its intima.