淋巴毒素A基因多态性与慢性阻塞性肺疾病并发骨质疏松的关系

Relationship between the lymphotoxin A gene polymorphism and complicating osteoporosis in patients with chronic obstructive pulmonary disease

目的探讨淋巴毒素A(LTA)基因多态性与北方汉族人群慢性阻塞性肺疾病(COPD)并发症骨质疏松的关系。方法收集2007年6-12月因COPD急性加重收住院治疗的122例COPD患者,在排除慢性肾功能不全以及甲状腺、甲状旁腺等疾病后,最后纳入101例COPD患者作为研究对象。采用双能X线骨密度测定仪测定患者的腰椎及股骨近端骨密度,根据骨密度情况将患者分为骨质疏松组(n=67)和非骨质疏松组(n=34)。以PCR直接测序法检测两组患者的6个LTA基因的tSNP位点(rs2844482,rs2071590,rs2239704,rs909253,rs2229094,rs1041981)的基因型分布,用χ2检验进行Hardy-Weinberg平衡检验,用非条件logistic回归模型计算比值比及95%可信区间,比较两组的差异。结果骨质疏松与非骨质疏松COPD患者LTA基因6个SNP位点的基因型分布频率均无显著性差异(P>0.05)。结论 LTA基因多态性与COPD并发骨质疏松可能无相关性。

Objective To explore the relationship between the lymphotoxin A （LTA） gene polymorphism and complicating osteoporosis in Hanzu patients with chronic obstructive pulmonary disease （COPD） living in northern China. Methods Of 122 patients with exacerbated COPD,admitted from June to December 2007,101 patients were finally selected as the subjects after ruling out those with chronic renal failure,and diseases of thyroid gland or parathyroid gland. Bone densitometry of patients＇ lumbar vertebrae and proximate femur was performed by X-ray absorptiometry. Based on the bone density,patients were assigned into osteoporosis group （n=67） and non-osteoporosis group （n=34）. Six sites of tag single nucleotide polymorphisms （tSNPs） （rs2844482,rs2071590,rs2239704,rs909253,rs2229094 and rs1041981） of LTA gene were selected from the 2 groups of patients for genotyping with polymerase chain reaction （PCR）-sequencing analysis. Hardy-Weinberg equilibrium was tested by chi-square analysis,95% confidential interval and odds ratio （RR） were calculated with non-conditional regression,and the differences between the 2 groups were then compared. Results The distribution frequency of genotypes of 6 tSNPs sites in COPD patients complicating with osteoporosis showed no significant difference to that in COPD patients without osteoporosis （P0.05）. Conclusion It seems that LTA gene polymorphism has no significant correlation with the risk of osteoporosis in patients suffering from COPD.