大鼠心理应激复合睡眠剥夺的营养干预实验研究

Effects of nutrition intervention on psychological stress combining with sleep deprivation in rats

目的探讨心理应激复合睡眠剥夺大鼠的营养干预效果。方法 75只雄性SD大鼠随机分为5组:空白对照组、复合因素组、复合对照组、营养一号组、营养二号组,每组15只。采用Communication Box和改良后的小平台法建立14d心理应激复合48h睡眠剥夺模型。营养一、二号组每天给予相应营养素灌胃,其余组给予等量生理盐水灌胃。利用Morris水迷宫进行大鼠定位航行实验以训练、检测大鼠的空间学习记忆能力,检测大鼠血清抗氧化能力和部分营养素指标。结果与复合因素组比较,营养一号干预后大鼠的各象限逃逸潜伏期缩短(P<0.05),谷胱甘肽过氧物酶(GSH-Px)活力和丙二醛(MDA)含量均增加(P<0.05),维生素E(Ve)、肌酸激酶(CK)活力和铁(Te)含量均增加(P<0.05);营养二号干预后大鼠的各象限逃逸潜伏期缩短(P<0.05),MDA含量增加(P<0.05),Ve含量增加(P<0.05),Vc含量降低(P<0.05)。与营养一号组比较,营养二号组的Ve含量增加(P<0.05),Vc含量降低(P<0.05)。结论营养干预对心理应激复合睡眠剥夺大鼠学习记忆、抗氧化能力、部分营养素指标均具有一定的改善作用;营养一号对部分指标的改善作用优于营养二号。

Objective To investigate the effect of nutrition intervention on psychological stress combining with sleep deprivation in rats. Methods Seventy-five male SD rats were randomly divided into five groups：control group,composite factor group,composite factor control group,nutrition group Ⅰ and nutrition group Ⅱ. The rat model of 14d psychological stress combining with 48h sleep deprivation was established by Communication Box and improved small platform methods. Rats in the 2 nutrition groups were gavaged with corresponding nutriment everyday; those in other groups were treated with gavage of physiological saline. Morris water maze place navigation test was employed to train rats and measure the ability of rats＇ spatial working memory. The antioxygenic potential of rats＇ blood serum and some nutrients were measured with corresponding kits. Results Compared with the composite factor group,the escape latency of all quadrants shortened significantly （P0.05）,both the activity of glutathione peroxidase （GSH-Px） and the contents of malondialdehyde （MDA） increased （P0.05）,and all the contents of vitamin E （Ve） and iron as well as the activity of creatine kinase （CK） increased obviously （P 0.05） in nutrition group Ⅰ; while in nutrition group Ⅱ,the escape latency of all quadrants was shortened significantly （P0.05）,the contents of MDA and Ve increased,and the Vc concentration decreased （P0.05）. Compared with nutrition group Ⅰ,the contents of Ve increased and that of Vc decreased in nutrition group Ⅱ （P0.05）. Conclusions Nutrition intervention may be beneficial in improving the abilities of spatial working memory and anti-oxidation of psychological stress combining with sleep deprivation in rats. The nutrition Ⅰ is better than nutrition Ⅱ on some of improvement index.