细胞组装体体外培养中的结构稳定性

Structure stability of cell assemblies for in vitro culturing

细胞组装体结构稳定性是体外培养的必要前提,凝胶溶胀和网状交联结构的破坏是影响稳定性的主要因素。结合戊二醛与壳聚糖明胶基共混材料的化学反应机理及交联剂扩散原理,采用质量分数为0.8%-1%的戊二醛溶液,交联15-60s,可实现明胶基质材料的细胞组装体体外培养稳定性。实验结果表明：用低质量分数戊二醛作为交联剂在细胞组装结构体外形成40-50μm保护层,保护层对细胞不呈毒性且具有降解性;同时能够保持结构体在较长培养时间内的宏观稳定性,适宜作为稳定性工艺试剂。

The structural stability of cell assemblies is a necessary prerequisite for in vitro culturing. Swell and damaged cross-linked net structures are the main factors influencing the stability. A stable cell and gelatin assembly for in vitro culturing was developed based on the chemical reaction and diffusion mechanics of a glutaraldehyde and hydrogel mixture composed of chitosan and gelatin, using a 08%-1% glutaraldehyde solution as the cross-linker and controlling the cross-linking time for 15-60 s. Tests show that the formatted 40-50 μm protective layer on the outside of cell assembly built using a low concentration of glutaraldehyde as a cross-linker, is not toxic to cells and can be degraded. The layer maintains the macro stability of the cell assembly during the long term incubation time. The low concentration glutaraldehyde solution is suitable as the process reagent for structure stability.