流动相对百树菊酯高效液相色谱手性分离的影响

The Effect of Mobile Phase on the Enantiomeric Separation of Cyfluthrin by Chiral High Performance Liquid Chromatography

在一种由N－3，5二硝基苯甲酸苯基甘氨酸衍生而成的Pirkle1-A型手性固定相上分离百树菊酯的8个立体异构体，通过选用不同的醇作为流动相的强溶剂，研究二元体系中流动相组分对保留值和选择性的影响。结果表明：对同一种组分的流动相，增加流动相的强度会缩短溶质的保留时间，且对非对映体的选择性影响比较大，而对对映体的选择性影响却不大，作为强溶剂的醇的结构不同会对异构体的分高度及峰形产生影响，二级醇或三级醇作强溶剂时的分高度好于一级醇，小分子醇作强溶剂时的峰形好于较大分子醇。

The enantiomeric separation of cynuthrin on chiral stationary phase (CSP), derived from (R)-N(3, 5-dinitrobenzoyl) phenylglycine (Prikle type 1-A CSP) has been studied by using different he-cane-alcohol mixtures as mobile phase. Mobile phase strength and composition have shown an important role in the chromatographic separation of eight isomers of cyfluthrin. Increase of the mobile phase strength reduces the retention of the eight isomers, while the diastereomeric selectivity is decreased and the enantiomeric selectivity remains almost unchanged. Also, the resolution varies in accordance with the steric hindrance of the alcohol.Secondary or tertiary alcohols impart a greater resolution to the CSP than primary alcohols. Peak shapes are better with the lower alcohols than the higher alcohols. For the enantiomeric separation of cyfluthrin on Pirkle type 1-A CSP, hexane-tert-butanol and hexane-2-propanol are better binary mobile phases.