免疫吸附治疗用ProteinA切流膜色谱柱的研究

Protein A Tangential Flow Membrane Chromatographic Column Used for Immunoadsorption Therapy

切流膜色谱柱是免疫吸附治疗中实现全血灌流的一种新的模式。实验研究表明，切流膜色谱柱的结构和血液流动形式对血细胞的损害很小。分别考察了水、血浆、血液等不同流体流速与切流膜色谱柱柱压降之间的关系，柱压降随着流体流速和粘度的升高而增高，血液流速为120mL／min时，柱压降达到93kPa；考察了ProteinA切流膜色谱柱对人血浆中免疫球蛋白IgG的吸附能力，切流膜色谱柱ProteinA健合量为139mg（6mgProteinA／g干介质），血浆在柱中循环1h可吸附IgG553mg（23．8mgIgG／g平介质），而血液在柱中循环1h,可吸附IgG499.4mm（21.5msIgG／g卡介质），循环时间对IgG吸附量影响较大，循环速度对IgG吸附量影响不大。对狗的体外临床血液灌流实验表明，切流膜色谱柱的血液相容性良好。

Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluidviscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0. 093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23. 8 mg IgG/g dry matrix) from human plasma and 499. 4 mg IgG (21. 5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity ; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood per fusion for dog showed that the Protein A column has good blood biocompatibility.