胶束电动毛细管色谱法分离左旋十八甲基炔诺酮、睾酮和孕酮

Separation of Levonorgestrel, Progesterone and Testosterone by Micellar Electrokinetic Capillary Chromatography

胶束电动毛细管色谱法（MECC）被用于左施十八甲基炔诺酮（LNC）、睾酮（T）和孕酮（P）的分离。电泳缓冲液中含一定量的乙腈或甲醇等有机添加剂时，可以改善分离。当乙睛体积分数为0～15％时，样品的迁移时间tm随着乙腈体积分数的增加而增加，但当已腈体积分数大于15％时，样品的tm随乙腈体积分数的增加反而减少。如采用20mmol／L的2，6-二甲基-β-环糊精（DM-β-CD）作添加剂，不仅可提高分高度，而且能缩短分析时间，实现最佳分离。

Separation of levonorgestrel, progesterone and testosterone was achieved by using micellar electrokinetic capillary chromatography (MECC). A column of 53 cm (to the detector) × 50μm i. d. uncoated fused-silica capillary (totally 68cm in length) and an ultra-violet detector with fixed wavelength at 254 nm were used throughout all the experiments. MECC analysis was optimized by evaluating three different micelle-forming agents, the concentration of SDS and several of organic additives in a 10 mmol/L borate running buffer (pH 9. 2). Complete separations were obtained with either 10%-20% acetonitrile or 20 mmol/L 2, 6-dimethyl- β -cyclodextrin (DM- β -CD)as the modifier in buffer. When the acetonitrile volume fraction was in the range of 0-15 %, the migration times of the three steroids increased with the acetonitrile volume fraction, but in the range of 15 %-20% acetonitrile concentrations, the situation was opposite. This behavior of the steroids was attributed to the interaction of two opposite effects, an increased mobility due to decreased partiton coefficient and a decreased electroosmotic flow (EOF). Both β-cyclodextrin (β-cD) and γ-cyclodextrin (γ- CD) were found to be inadequate for a satisfactory separation.