高效液相色谱法测定小鼠脑组织单胺递质及其相关化合物

Detennination of Monoamine Transmitters and Related Compounds in Mouse Brain by High Performance Liquid Chromatography With Fluorescence Detection

采用苯基柱等度洗脱分离,自然荧光检测法,直接测定小鼠脑组织匀浆中单胺递质及其相关化合物.优化出了最佳色谱条件,流动相为20mmol/L柠檬酸三钠(pH4.50)-甲醇(95:5,V/V);柱温为35℃.该方法能同时检测8种化合物.最低检测限为1.0～2.5μg/L;回收率均在93%以上;线性范围为1.0μg/L～1.0mg/L.

A rapid and simple isocratic elution chromatagraphic method for the determination of monoamine transmitters and related compounds using their native fluorescence by high performance liquid chromatography was developed. CLC-phenyl column was selected as separation column at 35℃, and 20 mmol/L of trisodium citrate(pH 4.50)-methanol (95:5, V/V) was isocratic eluted at flow rate of 1.0 mL/min. Eight compounds were simultaneously separated and determined. The recoveries of all compounds were above 93% , the detection limit was 1.0 μg/L- 2.5 μg/L and the linear range was 1.0 μg/L- 10 mg/L for compounds.