阿霉素纳米粒体外释放模型的拟合研究

A study on fitting models of the vitro release of Doxorubicin-nanoparticles

目的研究阿霉素溶液和阿霉素纳米粒体外释放行为的不同。方法:采用零级动力学方程、一级动力学方程、Higuchi方程和双相动力学方程拟合药物在体外的释放行为,根据拟合优度来判定曲线拟合情况,探讨方程所反应的释放机理。结果:阿霉素溶液在生理盐水中的体外释药用零级动力学方程、Higuchi方程和双相动力学方程拟合,拟合优度均不很好,而一级动力学方程则比较好。阿霉素溶液在体外的释放较快;阿霉素纳米粒在生理盐水中的释药情况用零级动力学方程、一级动力学方程和Higuchi方程描述时,拟合优度均不很好,而双相动力学方程则较好。阿霉素纳米粒在体外的释放较为缓慢。结论:阿霉素溶液体外释放速度较快,符合一级动力学方程;阿霉素纳米粒在体外的释放符合双相动力学方程。纳米粒在释药前由于纳米粒表面吸附有阿霉素,所以释药快,后来因为载体材料的溶解逐渐将阿霉素释放出来,这种释药规律符合抗癌药物的用药原则。

Objective Studying the property of nanoparticles and doxorubicin solution in vitro release were carried in this paper.Methods Several mathematical models of drug release,such as Zero order kinetic equation,First order kinetic equation,Higuchi equation and the double exponential diphase kinetics equation were used to fit the vitro release in normal saline of doxorubicin solution and doxorubicin nanoparticles,moreover,the best simulations for the vitro release of doxorubicin solution and doxorubicin nanoparticles were received.Results The first order model could fit doxorubicin solution release better than other equations,and the double exponential diphase kinetics equation model could fit doxorubicin nanoparticles better than other equations.The release of doxorubicin solution was faster than doxorubicin nanoparticles.Conclusion The consequence indicated that the release of doxorubicin nanoparticles was much better than doxorubicin solution.The first order was the best simulations for the vitro release of doxorubicin solution,and the double exponential diphase kinetics equation was the best simulations for the vitro release of doxorubicin nanoparticles.The release of doxorubicin nanoparticles was fast at the very start,because that the surface was accompanied by doxorubicin.And then relesed gradually as a result of the dissolution of carrier material.It is able to release slowly and the releasing properties in vitro of doxorubicin were consistent.