摘要
以甘蓝型油菜陇油2号子叶为转化受体材料,通过农杆菌介导将半夏凝集素抗虫基因(pta)导入陇油2号,经常规PCR检测获得阳性植株3株。以油菜磷酸烯醇式丙酮酸羧化酶基因(pep)作为内参基因、选pta全长序列中高度保守区域中145bp的pta2为目的片段对转基因植株进行REAL-TIMEPCR分析,初步证明外源pta基因已整合到油菜细胞基因组中。
Cotyledons with petiole from Brassica napus variety ＂Longyou2＂ were transformed by using Agrobacterium tumefaciens harbouring the plasmid with pta.Three positive transgenic plants were obtained.The endogenous pep gene and exogenous pta2 gene were amplified by Real-time PCR and the results confirmed that pta was integrated into the genome of Brassica napus.
出处
《作物杂志》
CAS
CSCD
北大核心
2010年第4期52-55,共4页
Crops
基金
甘肃省社会事业费项目(QS031-C31-15)
甘肃省农业科学院农业科技创新专项(2009GAAS17)
