二至丸对氧化性肝细胞损伤的保护作用研究

Protective Effect of Erzhi Pill on Oxidative Hepatocellular Injury

目的:研究二至丸对氧化性肝细胞损伤的保护作用。方法:采用过氧化氢体外诱导肝细胞氧化损伤,MTT法检测细胞活性,测定肝细胞上清总抗氧化能力(TAOC)、超氧化物歧化酶(SOD)的水平和丙二醛(MDA)的含量。结果:40、8μg·mL-1剂量的二至丸可显著提高肝细胞的TAOC和SOD水平,降低MDA含量,可明显抑制过氧化氢的氧化损伤,提高肝细胞总抗氧化能力。结论:二至丸可有效抑制肝细胞氧化损伤,该结论可为临床用药提供理论依据。

OBJECTIVE： To study the protective effect of Erzhi pill on oxidative hepatocellular injury. METHODS： The oxidative damage of hepatocytes was induced by H2O2 in vitro. The activity og hepatocytes was detected by MTT. The levels of TAOC and SOD and the content of MDA in hepatocytes were determined. RESULTS： The levels of TAOC and SOD were significantly improved by high-dose and middle-dose of Erzhi pill （40 μg·mL-1,8 μg·mL-1）. The content of MDA in hepatocytes was significantly decreased. High-dose and middle-dose of Erzhi pill could inhibit the oxidative damage of hepatocytes was induced by H2O2. Antioxidant ability of hepatocytes was improved. CONCLUSION： Erzhi pill could effectively inhibit oxidative hepatocellular injury, which provide theory evidence for clinical use of drugs.