摘要
目的:建立双黄解毒乳膏的质量标准。方法:采用薄层色谱法对制剂中的黄芩和关黄柏进行定性鉴别;采用高效液相色谱法对制剂中的黄芩苷含量进行定量分析。结果:定性鉴别方法能同时鉴别黄芩、关黄柏,专属性强,分离度好。黄芩苷进样浓度在80.0~400.0μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为97.3%,RSD=1.3%(n=9)。结论:所建标准可用于双黄解毒乳膏的质量控制。
OBJECTIVE: To establish the quality standard of Shuanghuang jiedu cream. METHODS: Scutellaria baicalensis and Phellodendron chinensis were identified qualitatively by TLC and the content of baicalin was determined by HPLC. RESULTS: The qualitative identification can be used to identify S. baicalensis and P. chinensis simultaneously. It is specific and well separated. The linear range of baicalin were 80.0 μg·mL-1 and 400.0 μg·mL-1(r=0.999 8)with an average recovery of 97.3% (RSD=1.3%,n=9). CONCLUSION: Established method be used for the quality control of Shuanghuang jiedu cream.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第31期2918-2920,共3页
China Pharmacy
基金
新疆维吾尔自治区科技攻关和重点科技项目资助课题(200333112B1)