铜摄入量对大鼠动脉粥样硬化炎症因子影响

Effect of copper intake on inflammatory factors associated with arteriosclerosis in rats

目的探讨铜摄入量对大鼠动脉粥样硬化(arteriosclerosis,AS)相关炎症因子的影响。方法选择Wistar大鼠饲以缺铜饲料并给予不同剂量葡萄糖酸铜30 d,以大鼠血中铜蓝蛋白(CP)为铜摄入量评价指标,分析其对大鼠AS相关炎症因子的影响。结果大鼠血中CP活力为20.442～37.569 U/L时,C反应蛋白(CRP)可对铜摄入量过高或过低引发的AS作出预警;铜摄入量低于或高于日常摄入量时,同型半胱氨酸(HCY)水平均高于对照组(P<0.05);CP活力超出30.279～37.569 U/L时,各组细胞间粘附因子-1(ICAM-1)水平均明显高于正常对照组(P<0.05);CP活力超出20.442～37.569 U/L时,各组单核细胞趋化因子-1(MCP-1)水平均高于正常对照组(P<0.05)。结论铜摄入量过高或过低均可不同程度激活AS相关各炎症因子,有引起AS的潜在风险。

Objective To find out the effect of copper intake on the inflammatory factors associated with arteriosclerosis（AS） formation in rats.Methods Wistar rats were fed with special feed without copper,and stock diet was given with copper gluconate at different dose with stomach tube every day for 30 days.Ceruloplasmin（CP） was used to study the effect of copper on the inflammatory factors associated with AS formation.Results When the activity of CP was in the range of 20.442-37.5692U/L,the C-reactive protein（CRP） could predict AS caused by abnormal copper intake.When the copper intake of the rats was lower or higher than normal daily intake,the level of homocysteine（HCY） in the blood of the rats was higher than normal level（P0.05）.The intercellular adhesion molecule-1（ICAM-1） level was higher than the normal level in the rats with abnormal copper intake with significant difference（P0.05） when the activity of CP was in the range of 30.279-37.569U/L.The monocyte chemotactic factor-1（MCP-1） level was higher than the normal level in all groups with a significant difference（P0.05） when the activity of CP was in the range of 20.442-37.569U/L.Conclusion The inflammatory factors associated with AS could be activated when the copper intake of rats were extremely high or low than the normal level.