麦麸多肽对小鼠抗氧化损伤作用

Antioxidation effect of wheat bran peptide on oxidative injury in mice

目的探讨麦麸多肽对氧化损伤模型小鼠的抗氧化作用。方法应用D-半乳糖建立亚急性衰老小鼠模型;麦麸多肽每d分别按150,300,600 mg/(kg.bw)的剂量灌胃,42 d后取脾脏及胸腺称重,计算脏器指数,测定血清、肝脏中丙二醛(MDA)含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果麦麸多肽300,600 mg/(kg.bw)组小鼠体重增长分别为(5.92±0.73),(6.19±0.30)g;脾脏指数分别为(4.39±0.06),(4.56±0.15);胸腺指数分别为(2.53±0.12),(2.96±0.11),与模型对照组比较,差异有统计学意义(P<0.01);血清和肝脏中MDA含量明显下降(P<0.01),SOD和GSH-Px活力明显提高(P<0.01)。结论在本实验条件下,麦麸多肽对氧化损伤模型小鼠有较强的抗氧化作用。

Objective To study the antioxidation effect of wheat bran peptides on D-galactose induced oxidative injury in mice.Methods Oxidative injury model in mice was induced by D-galactose.The mice in treatment groups were orally given wheat bran peptides at dose of 150,300,600 mg/kg·bw.The spleen and thymus gland were collected 42 days after the treatment to determine their indices.In addition,the malondialdehyde（MDA）contents,superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）activities in serum and liver were assayed.Results For mice in dose group of 300,600 mg/kg·bw,body weight gain were 5.92±0.73,6.19±0.30;the thymus index were 4.39±0.06,4.56±0.15;the spleen index were 2.53±0.12,2.96±0.11.The increases were all significant（P0.01）.The content of MDA significantly decreased and the activity of SOD and GSH-Px significantly increased both in serum and the liver of oxidative injury mouse model（P0.01）.Conclusion Wheat bran peptides have significant antioxidation effect on D-galactose induced oxidative injury in mice.