摘要
目的探讨麦麸多肽对氧化损伤模型小鼠的抗氧化作用。方法应用D-半乳糖建立亚急性衰老小鼠模型;麦麸多肽每d分别按150,300,600 mg/(kg.bw)的剂量灌胃,42 d后取脾脏及胸腺称重,计算脏器指数,测定血清、肝脏中丙二醛(MDA)含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果麦麸多肽300,600 mg/(kg.bw)组小鼠体重增长分别为(5.92±0.73),(6.19±0.30)g;脾脏指数分别为(4.39±0.06),(4.56±0.15);胸腺指数分别为(2.53±0.12),(2.96±0.11),与模型对照组比较,差异有统计学意义(P<0.01);血清和肝脏中MDA含量明显下降(P<0.01),SOD和GSH-Px活力明显提高(P<0.01)。结论在本实验条件下,麦麸多肽对氧化损伤模型小鼠有较强的抗氧化作用。
Objective To study the antioxidation effect of wheat bran peptides on D-galactose induced oxidative injury in mice.Methods Oxidative injury model in mice was induced by D-galactose.The mice in treatment groups were orally given wheat bran peptides at dose of 150,300,600 mg/kg·bw.The spleen and thymus gland were collected 42 days after the treatment to determine their indices.In addition,the malondialdehyde(MDA)contents,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities in serum and liver were assayed.Results For mice in dose group of 300,600 mg/kg·bw,body weight gain were 5.92±0.73,6.19±0.30;the thymus index were 4.39±0.06,4.56±0.15;the spleen index were 2.53±0.12,2.96±0.11.The increases were all significant(P0.01).The content of MDA significantly decreased and the activity of SOD and GSH-Px significantly increased both in serum and the liver of oxidative injury mouse model(P0.01).Conclusion Wheat bran peptides have significant antioxidation effect on D-galactose induced oxidative injury in mice.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第8期1050-1051,共2页
Chinese Journal of Public Health
基金
辽宁省教育厅项目(2009A811)
关键词
麦麸多肽
氧化损伤模型
抗氧化
wheat bran peptide
oxidative damage model
antioxidation