注射用七叶皂苷钠对大鼠局灶性脑缺血再灌注损伤后环氧合酶2表达的影响

Effect of aescinate on the expression of cyclooxygenase-2 in rats with focal cerebral ischemia reperfusion injury

目的:研究注射用七叶皂苷钠对大鼠局灶性脑缺血再灌注损伤后环氧合酶2(cyclooxygenase-2,COX-2)表达的影响。方法:健康雄性SD大鼠132只,随机分为假手术组、模型组、治疗组,后两组再分为再灌注6h、24h、48h、3d、5d亚组,每亚组12只大鼠。应用大脑中动脉线栓法建立大鼠局灶性脑缺血再灌注模型,造模成功治疗组即刻腹腔注射七叶皂苷钠5mg/kg/d,其余各组均腹腔注射等量生理盐水。各组大鼠于不同时间点分别评定神经功能缺损评分,免疫组织化学法检测脑组织COX-2的表达,TTC染色测定脑梗死体积。结果:注射用七叶皂苷钠能显著减少脑组织COX-2的表达,改善神经缺损症状,与各时间点模型组比较,P<0.05。结论:注射用七叶皂苷钠对局灶性脑缺血再灌注损伤具有保护作用,其作用机制可能与减少脑组织COX-2的表达有关。

Objective：To study the effect of aescinate on the expression of cyclcooxygenase-2 （COX-2） in rats with focal cerebral ischemia reperfusion injury.Method：One hundred and thirty-two healthy male Sprague-Dawley rats were randomly divided into sham operated group, model group and treatment group.The latter two groups were separately subdivided into reperfusion subgroups at the 6th h, 24th h, 48th h, 3rd d and 5th d.There were 12 rats in each subgroup.Rats were selected to establish focal cerebral ischemia reperfusion model by middle cerebral artery thread embolism method.After successful modeling, the treatment group was applied instantly with intraperitoneal injection with aescinate 5mg/kg/d, while the rest groups were given with intraperitoneal injection with equal volume of saline.The neurological deficit scores of rats in each group were respectively conducted at different time points.The COX-2 expression in brain tissue was detected by immunohistochemistry method and the infarct sizes were measured with TTC staining.Result：Aescinate could significantly reduce the expression of COX-2 in cerebral tissue and improve neurological symptoms comparing with model group at different time points（P0.05）.Conclusion：Aescinate played a protective role on focal cerebral ischemia reperfusion injury and the mechanism might be related to the reduction of COX-2 expression.