促红细胞生成素对急性心肌梗死家兔心室重构的影响

Effect of erythropoietin on myocardial remodeling after acute myocardial farction in rabbits

目的:探讨促红细胞生成素(EPO)对急性心肌梗死(AMI)家兔心室重构的影响。方法:开胸冠状动脉结扎法制备家兔AMI模型,随机分为假手术组、AMI对照组和EPO组,每组各10只。腹腔注射EPO5000U/kg,3次/周,24h后采血测心肌酶,4周后处死动物,获取心肌组织测定心室重量、心肌胶原含量及组织标本固定做病理分析。结果:与AMI组相比,EPO组的心肌酶水平、左心室重量(LVW)和右心室重量(RVW)、心肌胶原含量显著降低(P<0.05),心肌特殊染色可见左心室非梗死区AMI组变性的心肌细胞周围、血管周围胶原成分增生明显,而在EPO组胶原沉积比AMI对照组明显减轻。结论:EPO对AMI家兔有明显的心脏保护作用,抑制心室重构。

Objective：To explore the effect of erythropoietin （EPO）on myocardial remodeling after acute myocardial infarction in rabbits.Methods：By ligation of coronary artery acute myocardial infarction（AMI）model of rabbits was estabolished.Thirty rabbits were randomly divided into sham-operated group（n=10）,AMI control group（n=10）and EPO group（n=10）.The rabbits in EPO group received peritoneal injection EPO of 5000U/kg,three times per week,for four weeks.Blood samples were detected myocardial enzyme after 24h.Collagen of myocardium was measured and myocardium was fixed and pathologic analysis was performed.Results：Compared with AMI group,in EPO group,myocardial enzyme,left ventricular weight,right ventricular weight and collagen in noninfarcted were all significantly decreased （P0.05）.In the AMI group significant increased of collagen in noninfarcted of left ventricular around denatured myocardial cell and blood vessel was showed after particular staining.Collagen deposition was significantly decreased in EPO group compared with the AMI group. Conclusion：Erythropoietin had protective effects on myocardial remodeling after acute myocardial infarction in rabbits,reduced myocardial remodeling.