杜氏盐藻和亚心形扁藻在不同接种密度和氮浓度下的细胞群体生长(英文)

Colonial cell growth of Dunaliella salina and Platymonas subcordiformis under different inoculation density and nitrogen concentration

在氮浓度为1．4、14、和140mg·L^-1下，对杜氏盐藻（Dunaliella salina）和亚心形扁藻（Platymonas subcordiformis）进行细胞接种比例为10：0、7：3、5：5、3：7和0：10的培养试验，研究不同海洋经济微藻种间混合培养的细胞群体生长效应。结果表明，杜氏盐藻与亚心形扁藻细胞群体的生长随着氮浓度的增加而提高：两种藻在氮浓度分别为14和140mg·L^-1时混合培养的细胞群体生长明显优于单独培养。中、高氮下杜氏盐藻与亚心形扁藻以7：3的接种比例混合时，微藻的细胞群体生物量和胞内物质含量相比单独培养和其他比例均有显著提高，如叶绿素a含量比单独培养分别提高1．17倍和7．77倍；蛋白质含量比单独培养分别提高19．1％和195．3％等。而低氮浓度下，藻的生长受到氮浓度的限制。

We investigated the colonial cell growth of marine economic microalgae, Dunaliella salina and Platymonas subcordiformis under different cell inoculation densities （10 ： 0, 7 ： 3, 5 ： 5, 3 ： 7 and 0 ： 10） and nitrogen concentrations （low nitrogen： 1.4 mg·L^-1,medium nitrogen： 14 mg·L^-1 and high nitrogen： 140 mg·L^-1） by examining some parameters of algal cell density, biomass, chlorophyll a and protein content. The results indicated that colonial cell growth of these two algae increased with the increasing of nitrogen concentrations. Besides, cell growth of mixed-culture was superior to monoculture when at high and medium nitrogen concentrations; especially when they were cultured at a proportion of 7： 3. For example, chlorophyll a content was increased by as high as 1.17（p 〈 0.01） and 7.77 folds （p 〈 0.01）, and protein content was improved by 19.1%（ p 〈 0.05） and 195.3% （p 〈 0.01）, respectively. However, cell growth of mixed-culture was remarkably limited by nitrogen concentration when at low nitrogen concentration.