脾虚大鼠脑内IL-18蛋白及基因表达变化和益气扶正中药的干预研究

Changes of protein and gene expressions of brain IL-18 in spleen-deficiency rats and intervention of Chinese herbal remedies with actions of replenishing qi and reinforcing the healthy qi

目的研究脾虚模型脑内白细胞介素18(IL-18)蛋白及基因表达变化和益气扶正中药的干预作用。方法 40只大鼠随机分成4组,正常组、模型组、治疗1组(四君子汤)、治疗2组(玉屏风散),每组各10只。采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,采用称重法检测免疫器官指数,采用免疫组化法与原位杂交法检测下丘脑腹侧核、海马CA1区的IL-18蛋白表达与IL-18mRNA表达变化及四君子汤、玉屏风散的治疗作用。结果模型组胸腺及脾脏指数明显下降,模型组IL-18蛋白与IL-18mRNA表达在下丘脑腹侧核、海马CA1区明显降低;治疗组胸腺及脾脏指数增加,上述脑区的IL-18蛋白与IL-18mRNA表达明显增加。结论益气扶正中药四君子汤、玉屏风散可能通过影响免疫器官,调控细胞因子IL-18蛋白及基因表达而调节机体免疫功能。

Objective To study the changes of protein and gene expressions of brain IL-18 in spleendeficiency model and the interventional effects of Chinese herbal remedies with actions of replenishing qi and reinforce the healthy qi.Methods All rats（n=40） were randomly divided into the normal group,model group,treatment group 1（T group 1,treated with Sijunzi Decoction） and treatment group 2（T group 2,treated with Yupingfeng Powder,each n=10） .The spleen-deficiency model was established in rats by using the methods of purgation,improper diet and over strain.The indexes of immune organs were detected by applying weighing method.The changes of IL-18 protein and IL-18-mRNA expressions in the ventral nucleus of hypothalamus and CA1 zone of hippocampus and therapeutic effects of Sijunzi Decoction and Yupingfeng Powder were detected by applying immunohistochemistrical assay and in situ hybridization.Results In the model group the indexes of thymus and spleen,and IL-18 protein and IL-18-mRNA expressions all decreased significantly.In T group 1 and T group 2 the indexes of thymus and spleen,and IL-18 protein and IL-18-mRNA expressions all increased significantly.Conclusion Sijunzi Decoction and Yupingfeng Powder,with the actions of replenishing qi and reinforce the healthy qi,can regulate the immunologic function of the body through influencing immune organs and controlling the protein and gene expressions of brain IL-18.