茶淀红麦耐盐基因的RFLP分子标记

RFLP Molecular Marker of Salinity Tolerance Gene in Wheat Germplasm Chadian Red

以农家耐盐小麦品种“茶淀红麦”与盐敏感品种“中国春”为对象配制杂交组合,获得98株分离群体,利用BSA方法构建小麦耐盐和盐反应敏感基因池,利用RFLP探针标记茶淀红麦的耐盐基因。结果表明小麦7个同源群14个长短臂上的80个RFLP标记中仅有5A染色体长臂上的Psr906表现多态性,在32株F2群体(17耐和15感)表现共分离。由此将小麦耐盐种质茶淀红麦的耐盐基因定位在小麦遗传图谱5A染色体长臂的Xpsr906位点上。

Chadian Red, a slat tolerance wheat germplasm, was identified and crossed with salt sensitive genotype Chinese Spring(C. S). Two gene pools of salt tolerance and salt sensitivity was made from 98 individuals in F2 population by BSA method. RFLP was used to mark the salt tolerance genes. Only probe Psr906 was found to be polymorphic in total 80 RFLP probes locating on 7 homologous groups chromosome in wheat. The result showed that salt tolerance gene in Chadian Red was located on locus Xpsr906 of long arm of chromosome 5A in RFLP genetic mapping in wheat.