蜡样芽胞杆菌BC983的鉴定及生态效应的研究

Identificationof Bacilluscereus BC983 andEcologicalEffect

从健康鹿的粪便中分离到一株需氧的产芽胞革兰氏阳性大杆菌，经生理生化鉴定确定为蜡样芽胞杆菌（Ｂａｃｉｌｕｓｃｅｒｅｕｓ）。小鼠腹腔感染２５亿菌不致死。给育成鹿口服１０亿菌无异常反应。该菌的芽胞能耐受１００℃３０分钟煮沸。实验确定该菌在７％的氯化钠、４０％的牛胆汁中生长，生物夺氧试验表明，该菌能争夺环境中的氧，促进厌氧菌生长，用８亿活菌给育成鹿口服治疗大肠杆菌腹泻，治愈率为９１６％，据此，该菌可作为生态菌用于预防和治疗动物的细菌性腹泻病。

AnaerobiansporeformedgrampositivelargebacilluswasisolatedfromexcrementofhealthdeerByidentificationofphysiologicalandbiochemicalcharacteristics,itis BacilluscereusWTBZThemousesthatwereinfectedwith025billionbacteriabyabdominalcavitywerenotdeadBreedingdeerstakingonebillionorallywerenotabnormalreactionThesporeofthebacteriumcanresistboiling30minat100,theexperimentshowedthatthebacteriummaygrowin7%NaCland40%cowbile,theexperimentofbiologyscramblingoxygenshowedthebacteriumcanscrambletheoxygenofenvironmentandpromotedanaerobegrowth,08billionlivebacteriawereusedtocurediarrhoeaofEcolibacillusofbreedingdeers,cureratewas916%Accordingly,thebacteriummayregardasecologybacteriumtopreventandcurebacterialdiarrhoeadiseaseofanimals