唐山地区汉族人群白细胞介素6基因572C/G多态性与脑梗死关系的研究

Study on relation between interleukin-6 gene-572C/G polymorphisms and cerebral infarction in Tangshan Han population

目的探讨唐山地区汉族部分人群白细胞介素6(IL-6)基因-572C/G多态性与脑梗死的关系。方法采用多聚酶链反应-限制性片段长度多态性,检测118例首次新发脑梗死患者(脑梗死组)和154例健康体检者(对照组)IL-6基因-572C/G多态性位点频率,分析基因型与脑梗死的相关性。结果脑梗死组患者-572位点CC、CG、GG基因型频率分别为50.85%、41.53%、7.63%;对照组CC、CG、GG基因型频率分别为65.58%、31.82%、2.60%。脑梗死组和对照组-572位点C等位基因频率分别为71.61%、81.49%;G等位基因频率分别为28.39%、18.51%,差异有统计学意义(P<0.05)。CG、GG基因型人群患脑梗死的风险分别是CC基因型的1.683倍(95% CI:1.012～2.800,P<0.05)和3.788倍(95% CI:1.11 8～12.833,P<0.05)。多因素logistic回归分析显示,-572G等位基因是脑梗死发生的独立危险因素。结论唐山地区汉族部分人群中存在IL-6基因-572C/G多态性,可能与脑梗死有关,-572G等位基因可能是脑梗死发病的易感基因。

Objective To investigate the relationship between interleukin-6（IL-6） gene 572C/G polymorphisms and cerebral infarction in Tangshan Han population.Methods 118 old patients with cerebral infarction（CI） and 154 normal old people（control） were recruited.All participants were genotyped for the IL-6 gene at the 572C/G locus using PCRbased restriction fragment length polymorphism method.Results Genotype frequencies of IL-6 gene 572 CC,CG and GG were 50.85%,41.53% and 7.63% in patients with cerebral infarction and 65.58%,31.82% and 2.60% in control subjects.The C allele frequency was 71.61% in patients with CI and 81.49% in control subjects.The G allele frequency was 28.39% in patients with CI and 18.51% in control subjects.The frequencies of interleukin-6 gene 572 C/G genotypes and alleles in patients differed significantly from those in controls（P〈0.05）.The odds ratio（OR） for CI associated with CG,GG genotypes was estimated as 1.683（95%CI：1.012-2.800,P〈0.05） and 3.788（95%CI：1.118-12.833,P〈0.05）.Logistic regression analysis showed that IL-6 gene 572C/G polymorphism was an independent risk factor for cerebral infarction.Conclusion There is IL-6 gene 572C/G polymorphism in the test population.IL-6 gene 572C/G polymorphism may be a possible genetic susceptibility factor for cerebral infarction and 572G allele is associated with susceptibility to cerebral infarction in Tangshan Han population.