低酚陆地棉的体细胞胚胎发生和植株再生

Embryogenesis and Plant Regeneration from SomaticCells Culture of Glandless Upland Cotton

以生产上农艺性状表现良好的５个低酚陆地棉品种为材料对体细胞胚胎发生和植株再生进行了研究，供试５个品种均得到了再生植株，初步建立了低酚棉再生体系。研究结果表明，以无菌苗下胚轴为外植体，在附加ＩＡＡ，ＫＴ，２，４－Ｄ各０１ｍｇ／Ｌ的ＭＳ无机元素＋Ｂ５有机成分的培养基上，易诱导出灰软疏松的愈伤组织，当去掉２，４－Ｄ，且ＮＨ４ＮＯ３减半后，胚性愈伤组织大量出现，再除去激素，ＫＮＯ３加倍有利于胚状体的发生。在不含激素但含活性炭１５ｇ／Ｌ的ＳＨ培养基上，子叶期胚状体可以萌发，并长出具有根、茎、叶的完整植株。低酚棉再生体系的建立为棉花的基因转化奠定了基础。

Somatic embyrogenesis and plant regeneration in glandless upland cotton were studiedGray soft and loose callus could be initiatedeasily with hypocotyls of sterile plant in medium with inorganic ingredient of Ms+organic ingredient of B5+5710-7mol/L IAA+4610-7mol/L KT+4510-7mol/L 2,4-DLarge amount of embyrogenic callus were observed by discarding 2,4-D and half volume of NH4NO3Medium inorganic ingerdlent of Ms with double volume of KNO3+organic ingredient of B5 promoter embryo inductionCytoledon embryos could germinate into the complete plant with root,stem and leaf in medium SH without hormone+0125 mol/L active carbon