角质细胞生长因子对1C6细胞和4C18细胞的促增殖作用

Proliferative effect of keratinocyte growth factor on 1C6 cells and 4C18 cells

目的观察角质细胞生长因子(KGF)对胸腺上皮来源1C6细胞和4C18细胞的促增殖作用。方法鼠胸腺上皮髓质和皮质来源的1C6细胞、4C18细胞和鼠腹腔巨噬细胞来源的RAW细胞,分别经含不同浓度(25、50、100、200、400、800ng/ml)的KGF和不含KGF的完全培养液(对照组)作用24、48和72h,采用噻唑蓝(MTT)法测定细胞增殖率,并绘制细胞增殖率曲线。结果 MTT法检测显示,在低浓度(<200ng/ml)时,1C6细胞增殖率随KGF浓度增加不断升高;KGF浓度为200ng/ml时,1C6细胞增殖率达最高值;在高浓度(>200ng/ml)时,1C6细胞增殖率随KGF浓度的增加逐渐下降;与对照组比较,浓度为100、200、400ng/ml的KGF分别作用24、48和72h对1C6细胞均具有明显的促增殖作用(均P<0.05)。在低浓度(<100ng/ml)时,4C18细胞增殖率随KGF浓度增加不断升高;KGF浓度为100ng/ml时,4C18细胞增殖率达最高值;在高浓度(>100ng/ml)时,4C18细胞增殖率随KGF浓度的增加逐渐下降;与对照组比较,浓度为50、100、200、400ng/ml的KGF分别作用24、48和72h对4C18细胞均具有明显的促增殖作用(均P<0.05)。但不同浓度的KGF对RAW细胞均未见促增殖作用。结论 KGF对胸腺上皮来源的1C6细胞和4C18细胞均具有明显的促增殖作用。

Objective To observe proliferative effect of keratinocyte growth factor （KGF） on 1C6 cells and 4C18 cells originated from thymic epithelial cell. Methods 1C6 cells and 4C18 cells originated from the medulla and cortex of mouse thymic epithelial,and RAW cells from mouse peritoneal macrophage were cultured in complete culture medium with different concentrations （25,50,100,200,400,800 ng/ml） of KGF and without KGF （control group） for 24,48 and 72 h respectively,and cell proliferation rate was analyzed by MTT assay,at the same time proliferation curves were made. Results MTT assay showed that when KGF concentration was less than 200 ng/ml,the proliferation rate of 1C6 cells increased as the concentration raising,when KGF concentration was 200 ng/ml,the proliferation rate of 1C6 cells reached the peak value,and when KGF concentration was more than 200 ng/ml,the proliferation rate of 1C6 cells decreased gradually as the concentration declining. Compared with the control group respectively,the proliferative effects on 1C6 cells affected by concentrations of 100,200,400 ng/ml of KGF for 24,48 and 72 h were obvious （all P 0.05）. When KGF concentration was less than 100 ng/ml,the proliferation rate of 4C18 cells increased as the concentration raising,when KGF concentration was 100 ng/ml,the proliferation rate of 4C18 cells reached the peak value,and when KGF concentration was more than 100 ng/ml,the proliferation rate of 4C18 cells decreased gradually as the concentration declining. Compared with the control group respectively,the proliferative effects on 4C18 cells affected by concentrations of 50,100,200 and 400 ng/ml of KGF for 24,48 and 72 h were obvious （all P 0.05）. But different concentration of KGF had no proliferation effect on RAW cells. Conclusion KGF could promote the proliferation of 1C6 cells and 4C18 cells originated from thymic epithelial cells.