低氧诱导因子-1基因在宫颈癌组织中的表达及意义

Expression of Hypoxia induciblefactor-1 Gene in cervical cancer and its clinical significance

目的研究HIF-1在宫颈癌组织、正常宫颈组织及宫颈良性病变组织中的表达,并与宫颈癌患者年龄、淋巴结转移与否、临床分期进行比较,探讨其与宫颈癌侵袭/转移的关系。方法应用逆转录聚合酶链式反应(RT-PCR)的方法检测56例宫颈癌组织、正常宫颈组织及20例宫颈良性病变组织中HIF-1mRNA的表达情况,并应用相关统计学方法进行分析。结果 HIF-1mRNA在宫颈癌组织、正常宫颈组织及宫颈良性病变组织中的表达率分别为62.50%、41.07%、35.00%,宫颈癌与正常宫颈组织(P=0.023)、宫颈癌与宫颈良性病变组织(P=0.001)间的HIF-1αmRNA表达率差异有统计学意义,而正常宫颈组织与宫颈良性病变组织之间的HIF-1αmRNA表达率差异无统计学意义(P=0.091)。HIF-1mRNA在不同临床分期(P=0.001)及淋巴结转移(P=0.002)中的表达率存在显著差异,但在不同年龄患者的癌组织中HIF-1mRNA的表达率无差异(P>0.05)。结论 HIF-1mRNA在宫颈癌中的表达率明显高于正常宫颈组织及宫颈良性病变组织。HIF-1与宫颈癌临床分期密切相关,临床分期越晚,其肿瘤组织中HIF-1mRNA的表达率越高。伴淋巴结转移宫颈癌HIF-1mRNA表达率高于无淋巴结转移者。HIF-1mRNA表达率与患者年龄无关。

Objective To study the expression of HIF-1 in human cervical cancer and explore the relationship between HIF-1expression and clinical pathological features（ patient’s age,limph node metastasis,clinical stage,） . Methods The expression of HIF-1 mRNA in 56 cases of human cervical cancer tissues,normal cervical tissues and 20 cases of cervical benign lesion tissues was detected by reverse transcriptase polymerase chainreaction （ RT-PCR） . Results The expression rate of HIF-1 mRNA in cervical cancer,normal cervical tissues and cervical benign lesion tissue were 62. 50% 、41. 07% 、35. 00% ,statistical difference was found among cervical carcinoma group,normal cervical tissues group and benign lesion group. The expression of HIF-1 mRNA were associated with clinical stage （ P = 0. 001） and lymph node metastasis （ P = 0. 002） ,but had no correlation with age and tumor size （ P 0. 05） . Conclusion HIF-1 mRNA expression is increased in cervical cancer tumor tissues compared with normal tissues and cervical benign lesion tissue,HIF-1 is relevant with the occurrence of cervical cancer. The cervical cancer cells proliferation capacity is increase,degree of malignancy is higher and facilitates metastasis occurred which HIF-1 is expressed.