γ-干扰素基因多态性与肺癌易感性的关系研究

Study on the Correlation between the Polymorphism of Interferon -γGene and Susceptibility to the Development of Lung Cancer

目的 研究γ-干扰素基因（IFN-γ）（＋874 T/A）多态性与肺癌易感性的关系.方法 利用序列特异性引物多聚酶链反应（PCR-SSP）技术对42例肺癌患者及60例对照人群（IFN-γ）（＋874T/A）多态性进行检测,并对其易感性进行分析.结果 对照组与肺癌组AA基因型频率分别为0.533、0.786,对照组显著小于肺癌组（P＜0.01）,而AT、TT基因型频率显著大于肺癌组（P＜0.01,P＜0.05）.肺癌组A等位基因频率（0.869）显著高于对照组（0.7）（P＜0.01）,相对危险度（RR）为2.84,95%可信限（95%CI）为1.31～6.15,P＜0.01.结论 IFN-γ（＋874T/A）多态性多态性可能与肺癌的易感性相关,因此其多态性检测可作为遗传标记之一进行检测,用于早期发现对癌易感的高风险人群.

Objective To study the correlation between the polymorphism of Interferon γ（ IFN-γ） （ ＋ 874 T/A） gene and susceptibility to the development of lung cancer. Methods The polymorphism of IFN-7 gene（ ＋ 874 T/A） in 42 patients with lung cancer and 60 healthy control subjects was detected by using sequence-specific primers polymerase chain reaction（ PCR-SSP） and their susceptibility to development of lung cancer was analyzed. Results The genotypic frequencies of AA of IFN -γ Gene（ ＋ 874 T/A） in the control group and the patient group were 0. 533, 0.786 .respectively and the genotypic frequency of AA in control group was significantly lower than that in the patient group （ P 〈 0. 01） , in contrast with which the genotypic frequencies of AT and TT in the control group were significantly higher than those in the patient group （P 〈 0. 01 ,P 〈 0.05）. The A allele frequency of IFN-γ gene in the patient group was statistically higher compared with that in the control group（0. 869 vs. 0.7 ,P 〈0.01 ） ,in which the relative riskrate（RR） was 2.84,95%CI 1. 31 ~6.15 ,P〈0. 01. Conclusion The polymorphism of IFN-γ gene （ ＋874 T/ A） could be associated with susceptibility to the development of lung cancer,so it could be considered one of the genetic markers to be used to early identify individuals at high risk of lung cancer.