响应面法优化超临界CO_2萃取栝楼籽油

Optimization of Supercritical CO_2 Extraction of Trichosanthes kirilowii Maxim.Seed Oil by Response Surface Methodology

通过响应面法(response surface methodology)优化超临界二氧化碳萃取栝楼籽油工艺,采用DesignExpert软件对试验数据进行分析,气相色谱-质谱(gas chromatography-mass spectrometry)对萃取栝楼籽油进行成分分析。结果表明,最佳工艺参数为萃取压力29.75 MPa,萃取温度45.1℃,萃取时间为175.8min,栝楼籽油萃取得率为32.82%,栝楼籽油主要成分为棕榈酸(7.90%)、α-亚麻酸(28.23%)、亚油酸(39.33%)和油酸(22.57%)为主,另外检出不饱和脂肪酸7-棕榈烯酸(0.40%)、γ-亚麻酸(0.22%)和11-二十碳烯酸(0.30%),同时检出不饱和烃类角鲨烯(0.33%)。萃取时间、萃取压力、萃取温度对栝楼籽油超临界CO_2萃取工艺有显著的影响,栝楼籽油不饱和脂肪酸质量分数达90.59%。

The process variables for the supercritical carbon dioxide（SC - CO_2） extraction of Trickosanthes kirilowii Maxim seed oil was optimized by response surface methodology,and the experimental data were analyzed with Design Expert software.The composition of the crud oil was identified by gas chromatography - mass spectrometry. Results：The obtained optimum process parameters are pressure 29.75 Mpa,temperature 45.1℃,and time 175.8 min.Under the conditions,the oil yield reaches 32.82%,consistent with measured values.The extracted oil is composed of saturated fatty acids 9.41%and unsaturated fatty acids 90.59%.The abundant fatty acid components are oleic acid 22.57%,linoleic acid 39.33%,α-linolenic acid 28.23%,and palmitic acid 7.90%,additionally,7 - palmitoleic acid 0.40%,gamma - linolenic acid 0.22%,11 - eicosenoic acid 0.30%and squalene 0.33%are also detected.The optimized supercritical extraction technique for Trichosanthes kirilowii Maxim.seed oil is feasible.