丹皮总苷体外对三类免疫细胞功能的影响

Effects of total glucosides of mudan cortex on functions of three types of immune cells in vitro

目的研究丹皮总苷（ＴＧＭ）体外对３类免疫细胞功能的影响。方法采用［３Ｈ］－ＴｄＲ参入法，检测Ｔ淋巴细胞增殖反应和分泌白介素２（ＩＬ－２）活性，Ｂ淋巴细胞增殖反应以及巨噬细胞产生ＩＬ－１。结果ＴＧＭ２～５０ｍｇ·Ｌ－１可明显促进刀豆蛋白Ａ诱导小鼠Ｔ淋巴细胞增殖反应和大鼠Ｔ淋巴细胞产生ＩＬ－２，ＴＧＭ还可促进脂多糖诱导Ｂ淋巴细胞增殖反应以及大鼠腹腔巨噬细胞产生ＩＬ－１，它们的浓度－效应曲线呈钟罩形。结论ＴＧＭ具有浓度依赖性双向免疫调节作用。

AIM To study the effects of total glucosides of mudan cortex (TGM) on functions of three types of immune cells in vitro . METHODS T lymphocyte proliferation and its interleukin 2 (IL 2) secretion, B lymphocyte proliferation, IL 1 production by peritoneal macrophages were measured by TdR incorporation. RESULTS TGM 2￣50 mg·L -1 could significantly promote murine T lympocyte proliferation and IL 2 secretion by rat T lymphocyte induced by concanavalin A, and murine B lymphocyte proliferation as well as IL 1 production of rat peritoneal macrophages induced by lipopolysaccharide. Their concentration effect curves showed the bell shaped. CONCLUSION TGM has a dual immunomodulatory activity in a concentration related way.