大肠癌和卵巢癌病人微卫星序列变化研究

Microsatellite Instabilities in Colorectal and Ovarian Cancers

目的：研究大肠癌和卵巢癌病人基因组微卫星ＤＮＡ序列变化。方法：应用ＰＣＲ、聚丙烯酰胺凝胶电泳和同位素技术检测３５例大肠癌和１５例卵巢癌病人基因组３个微卫星位点变化。结果：１０例大肠癌（２８．５７％）和３例卵巢癌（２０％）存在至少一个微卫星位点的变化。结论：部分大肠癌和卵巢癌病人表现微卫星ＤＮＡ不稳定，与其发生机理有关。

Objective: To study the microsatellite DNA changes in the genome of patients with colorectal and ovarian cancers. Methods: Polymerase chain reaction, polyacrylamide electrophoresis and isotope were used to detect microsatellite instability (MIN) in 35 colorectal and 15 ovarian cancers. Results: MIN of at least one marker existed in 28.57% (10/35) patients with colorectal cancers and in 20% (3/15) patients with ovarian cancers. Conclusion: A subset of colorectal and ovarian cancers showed MIN, which is related to their oncogenesis.