兔视网膜色素上皮细胞的体外培养及超微结构研究

In Vitro Culture and Ultrastructure of Rabbit Retinal Pigment Epithelial Cells

目的：将兔视网膜色素上皮（ＲＰＥ）细胞进行体外培养，观察其超微结构。方法：用胰酶消化法培养兔ＲＰＥ细胞并传代。扫描电镜和透射电镜观察其超微结构。结果：原代培养的ＲＰＥ细胞含大量色素呈多角形，传代的细胞透明呈梭形。电镜显示它们具有细胞极性，含黑色素颗粒、各种细胞器、细胞间连接复合体，与体内一致。结论：培养的大量细胞可用于ＲＰＥ的体外实验研究。

Objective: We were to establish the culture of rabbit retinal pigment epithelial (RPE) cells and observe their ultrastructure. Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro. The ultrastructure of RPE was observed by scaning and transmission electron microscopy. Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape. Electron microscopy demonstrated that the normal morphological characteristics of rabbit RPE in vivo were maintained in cultured cells, which included cell polarity, pigments, intracellular organelles, and cell junction complex. Conclusion: This technique allowed us to produce a large quantity of RPE cells for in vitro experiments.