血小板蛋白激酶C在脑梗塞中致病作用的研究

Study on the Role of Protein Kinase C in Platelets in Cerebral Infarction

目的：研究血小板蛋白激酶Ｃ（ＰＫＣ）在脑梗塞中的活性，以揭示ＰＫＣ的致病机理。方法：利用３２Ｐ－ＮａＨ２ＰＯ４标记血小板，以ＰＫＣ的４０ｋｄ、２０ｋｄ底物的磷酸化作为酶激活的标志，对脑梗塞组及正常对照组ＰＫＣ的基础活性及反应活性经ｔ检验进行分析比较。结果：ＰＫＣ的基础活性及经凝血酶刺激后的反应活性均较对照组显著升高（Ｐ＜０．０１）。结论：脑梗塞中血小板ＰＫＣ的异常活化及高反应性在血小板血栓形成中起重要作用。

Objective:To study the protein kinase C (PKC) activity in platelets for the patients with cerebral infarction and its role in the disease. Methods:We labelled platelates with 32 P NaH 2PO 4 and considered the mark of activated PKC was the phosphorylation of platelet membrane 40 kd, 20 kd substrate. We compared and analysed the basic and responsive activity of platelet PKC between cerebral infarction group and control group by t test. Results:The basic activity and responsive activity of platelet PKC after thrombin's stimulation in patient group were remarkably elevated than in control group (P<0.01). Conclusion: The unusual activated PKC and its high responsiveness played important roles in platelet thrombosis for the patients with cerebral infarction.