婴幼儿血管瘤中bFGF与细胞外基质相关关系的研究

The Correlation Between Basic Fibroblast Growth Factor and Extracellular Matrix in Infantile Hemangiomas

目的：通过检测碱性成纤维细胞生长因子Ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）与Ⅳ型胶原（ＩＶｔｙｐｅｃｏｌａｇｅｎ，ＩＶ－ｃｏｌ）、层粘连蛋白（Ｌａｍｉｎｉｎ，ＬＮ）在婴幼儿血管瘤中的表达，探讨它们与血管瘤发病机理的关系。方法：采用ＳＰ免疫组化法和图像分析技术，对７０例婴幼儿血管瘤组织中的ｂＦＧＦ、ＩＶ－ｃｏｌ和ＬＮ进行了定性和定量分析。结果：ｂＦＧＦ位于胞浆，胞核染色较淡；但主要位于细胞外基质，呈线网状分布。ＩＶ－ｃｏｌ，ＬＮ主要位于小血管及增生的腺管周围，连续线状分布。三者在血管瘤组织中均呈高表达，且ｂＦＧＦ与ＩＶ－ｃｏｌ、ＬＮ之间相关关系显著。结论：ｂＦＧＦ和细胞外基质的高表达与血管瘤的发生、发展密切相关。

Objective:This paper was to detect the expression of basic fibroblast growth factor (bFGF), type IV collagen(IV col),and Laminin(LN)in infantile hemangiomas for probing the pathogenesis of hemangiomas. Methods: We analysed the bFGF,IV col,and LN qualitatively and quantitatively in 70 cases of infantile hemangiomas,useing immunohistochemical SP method and image analysis technique. Results: There was obvious overexpression of bFGF, IV col,and LN in hemangiomas. bFGF was present in the cytoplasm of endotheliocytes, mainly in extracellular matrix(ECM),and manifested in linear reticulation.IV col and LN were primarily situated around the small vessels and adenic canals in hemangiomas with continuous linear contribution. bFGF was closely correlated with IV col and LN.Conclusion:Overexpression of bFGF,IV col,and LN in hemangiomas might be related to the occurrence and progression of infantile hemangiomas.