放疗前后宫颈癌中Survivin表达及凋亡研究

Study the expression and apoptosis of surviving pre-and post-radiotherapy in cervical carcinoma

目的研究放疗前后宫颈癌组织Survivin表达水平及凋亡,探讨其与放疗效果的相关性。方法选取38例接受放疗的宫颈癌患者,用免疫组化法分别检测放疗前后Survivin的表达及TUNEL法检测细胞凋亡的情况,并评价放疗效果。结果 Survivin表达阴性和阳性相比,前者的放疗效果优于后者,差异有统计学意义(P<0.05);放疗后获得临床稳定的患者与获得临床部分缓解的患者相比,Survivin上调的比例明显高于后者,差异有统计学意义(P<0.05);放疗后Survivin阴性表达者的细胞凋亡明显增多,差异有统计学意义(P<0.05)。结论 Survivin基因有望成为宫颈癌逆转放疗抵抗的新靶点。

Objective To study the correlation between the expression of survivin or its change after radiotherapy and effect in cervical carcinoma.Methods 38 cases of women with cervical carcinoma were treated with radiotherapy.The expression of Survivin pre-and post-radiotherapy was measured by immunohistochemically; and cell apoptosis were examined by TUNEL method,and the re-sponse to the treatment was evaluated.Results The effect of patients with negative survivin of radiotherapy presented better than those with intense Survivin,and difference was significant （ P0.05） .Among the patients who were no change,the expression of Survivin was up-regulated more significantly after radiotherapy than those with partial clinical release,and the difference was significant （ P0.05） ; apoptosis cells with negative Survivin expressed after radiotherapy were increased,and the difference was also significant （ P0.05） .Conclusions Survivin gene may be new target to reverse radiotherapy resist.